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Anti-Cystatin F Antibody (A10321)

Western Blot - Anti-Cystatin F Antibody (A10321) - Antibodies.com Immunofluorescence - Anti-Cystatin F Antibody (A10321) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Cystatin F for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Cystatin F Antibody
Description
Rabbit polyclonal antibody to Cystatin F.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human CST7 (NP_003641.3).
Sequence
GPSPDTCSQDLNSRVKPGFPKTIKTNDPGVLQAARYSVEKFNNCTNDMFLFKESRITRALVQIVKGLKYMLEVEIGRTTCKKNQHLRLDDCDFQTNHTLKQTLSCYSEVWVVPWLQHFEVPVLRCH
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
16 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CMAP, CST 7, Cst7, Cystatin 7, cystatin F (leukocystatin), Cystatin like metastasis associated protein, Cystatin-7, Cystatin-F, Cystatin-like metastasis-associated protein, Cystatin7, CystatinF, CYTF_HUMAN, dJ568C11.1, Leukocystatin
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Cystatin F Antibody (A10321) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Cystatin F Antibody (A10321)
Western blot analysis of extracts of various cell lines, using Anti-Cystatin F Antibody (A10321) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunofluorescence - Anti-Cystatin F Antibody (A10321) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Cystatin F Antibody (A10321)
Immunofluorescence analysis of mouse spleen using Anti-Cystatin F Antibody (A10321) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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