Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CYB 5, CYB 5A, CYB5, Cyb5a, CYB5_HUMAN, Cytochrome b 5, Cytochrome b5 (microsomal), Cytochrome b5 type A, Cytochrome b5 type A (microsomal), MCB 5, MCB5, Microsomal cytochrome b5, Microsomal cytochrome b5 type A, Type 1 cyt b5
Figure 1: Western Blot - Anti-Cytochrome b5 Antibody (A14735)
Western blot analysis of extracts of various cell lines, using Anti-Cytochrome b5 Antibody (A14735) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 120s.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Cytochrome b5 Antibody (A14735) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-Cytochrome b5 Antibody (A14735) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using Anti-Cytochrome b5 Antibody (A14735) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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