Anti-Cytochrome P450 2E1 Antibody [ARC58001] (A306497)

Name: Anti-Cytochrome P450 2E1 Antibody [ARC58001]
Brand: Antibodies.com
SKU: A306497

Enlarge Image

This product is discontinued

Anti-Cytochrome P450 2E1 Antibody [ARC58001] (A306497) has been discontinued and is no longer available.

View all Anti-Cytochrome P450 2E1 Antibodies.

Specifications

Name

Anti-Cytochrome P450 2E1 Antibody [ARC58001]

Description

Rabbit monoclonal [ARC58001] antibody to Cytochrome P450 2E1.

Applications

WB, ICC/IF

Dilutions

WB: 1:2,000-1:10,000, ICC/IF: 1:50-1:200

Reactivity

Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 234-493 of human Cytochrome P450 2E1 (CYP2E1) (NP_000764.1).

Sequence

KVIKNVAEVKEYVSERVKEHHQSLDPNCPRDLTDCLLVEMEKEKHSAERLYTMDGITVTVADLFFAGTETTSTTLRYGLLILMKYPEIEEKLHEEIDRVIGPSRIPAIKDRQEMPYMDAVVHEIQRFITLVPSNLPHEATRDTIFRGYLIPKGTVVVPTLDSVLYDNQEFPDPEKFKPEHFLNENGKFKYSDYFKPFSTGKRVCAGEGLARMELFLLLCAILQHFNLKPLVDPKDIDLSPIHIGFGCIPPRYKLCVIPRS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC58001

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

57 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

4-nitrophenol 2-hydroxylase, CP2E1_HUMAN, CPE1, CYP2E, Cyp2e1, CYPIIE1, Cytochrome P450 isozyme 3A, Cytochrome P450 LM3A, Cytochrome P450, family 2, subfamily e, polypeptide 1, Cytochrome P450, subfamily IIE, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, Cytochrome P450-ALC, Cytochrome P450-J, Cytochrome P450RLM6, EC 1.14.13.-, EC 1.14.13.n7, Ethanol-inducible P450, Flavoprotein-linked monooxygenase, MGC133529, Microsomal monooxygenase, OTTHUMP00000020813, OTTHUMP00000046571, P450 ALC, P450-J, P450C2E, P450J, P450RLM6, Xenobiotic monooxygenase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

Enlarge Image

Figure 1: Western Blot - Anti-Cytochrome P450 2E1 Antibody [ARC58001] (A306497)

Western blot analysis of Mouse kidney, using Anti-Cytochrome P450 2E1 Antibody [ARC58001] (A306497) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Figure 2: Immunofluorescence - Anti-Cytochrome P450 2E1 Antibody [ARC58001] (A306497)

Immunofluorescence analysis of mouse kidney using Anti-Cytochrome P450 2E1 Antibody [ARC58001] (A306497) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Publishing research using Anti-Cytochrome P450 2E1 Antibody [ARC58001] (A306497)? Please let us know so that we can list the citation on this page.