Anti-Cytokeratin 13 Antibody (A91859)

Name: Anti-Cytokeratin 13 Antibody
Brand: Antibodies.com
SKU: A91859
Price: 335.0 USD
Availability: InStock

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This product is discontinued

Anti-Cytokeratin 13 Antibody (A91859) has been discontinued and is no longer available.

View all Anti-Cytokeratin 13 Antibodies.

Specifications

Name

Anti-Cytokeratin 13 Antibody

Description

Rabbit polyclonal antibody to Cytokeratin 13.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 269-458 of human Cytokeratin 13 (Cytokeratin 13 (KRT13)) (NP_705694.2).

Sequence

DLTRVLAEMREQYEAMAERNRRDAEEWFHAKSAELNKEVSTNTAMIQTSKTEITELRRTLQGLEIELQSQLSMKAGLENTVAETECRYALQLQQIQGLISSIEAQLSELRSEMECQNQEYKMLLDIKTRLEQEIATYRSLLEGQDAKMIGFPSSAGSVSPRSTSVTTTSSASVTTTSNASGRRTSDVRRP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

47 kDa cytokeratin, CK-13, CK13, Cytokeratin-13, K13, K1C13_HUMAN, Ka13, Keratin, Keratin 13, keratin type I cytoskeletal 13, Keratin-13, Krt-1.13, Krt1-13, KRT13, MGC161462, MGC3781, type I cytoskeletal 13, Type I keratin Ka13, WSN2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Cytokeratin 13 Antibody (A91859)

Western blot analysis of extracts of A-431 cells, using Anti-Cytokeratin 13 Antibody (A91859) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-Cytokeratin 13 Antibody (A91859)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cytokeratin 13 Antibody (A91859) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Cytokeratin 13 Antibody (A91859)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Cytokeratin 13 Antibody (A91859) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Cytokeratin 13 Antibody (A91859)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-Cytokeratin 13 Antibody (A91859) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.