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Anti-Cytokeratin 19 Antibody [ARC2811] (A307943)

Western Blot - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) - Antibodies.com Immunofluorescence - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC2811] antibody to Cytokeratin 19 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Cytokeratin 19 Antibody [ARC2811]
Description
Rabbit monoclonal [ARC2811] antibody to Cytokeratin 19.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 19 (KRT19) (KRT19) (P08727).
Sequence
MTSYSYRQSSATSSFGGLGGGSVRFGPGVAFRAPSIHGGSGGRGVSVSSARFVSSSSSGAYGGGYGGVLTASDGLLAGNEKLTMQNLNDRLASYLDKVRA
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2811
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
43 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
40 kDa keratin intermediate filament, CK 19, CK-19, CK19, Cytokeratin-19, K19, K1C19_HUMAN, K1CS, Keratin 19, Keratin type I 40 kD, Keratin type I 40kD, Keratin type I cytoskeletal 19, Keratin, type I cytoskeletal 19, Keratin, type I, 40 kd, Keratin-19, KRT19, MGC15366
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943)
Western blot analysis of various lysates, using Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 0.5s.
Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943)
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC2811] (A307943)
Immunohistochemistry analysis of paraffin-embedded human hepatocellular carcinoma (negative control sample) using Anti-Cytokeratin 19 Antibody [ARC2811] (A307943) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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