Anti-Cytokeratin 2e Antibody [ARC1925] (A309233)

Name: Anti-Cytokeratin 2e Antibody [ARC1925]
Brand: Antibodies.com
SKU: A309233
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1925] antibody to Cytokeratin 2e for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Cytokeratin 2e Antibody [ARC1925]

Description

Rabbit monoclonal [ARC1925] antibody to Cytokeratin 2e.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 293-422 of human Cytokeratin 2e (KRT2) (P35908).

Sequence

YMIKVELQSKVDLLNQEIEFLKVLYDAEISQIHQSVTDTNVILSMDNSRNLDLDSIIAEVKAQYEEIAQRSKEEAEALYHSKYEELQVTVGRHGDSLKEIKIEISELNRVIQRLQGEIAHVKKQCKNVQD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1925

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CK 2e, Cytokeratin-2e, Epithelial keratin-2e, K2e, Keratin type II cytoskeletal 2 epidermal, Keratin-2 epidermis, KRT2, KRT2A, KRT2E, Type-II keratin Kb2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Cytokeratin 2e Antibody [ARC1925] (A309233)

Western blot analysis of extracts of A-431 cells, using Anti-Cytokeratin 2e Antibody [ARC1925] (A309233) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-Cytokeratin 2e Antibody [ARC1925] (A309233)

Immunohistochemistry analysis of paraffin-embedded mouse skin using Anti-Cytokeratin 2e Antibody [ARC1925] (A309233) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Cytokeratin 2e Antibody [ARC1925] (A309233)

Immunofluorescence analysis of rat skin using Anti-Cytokeratin 2e Antibody [ARC1925] (A309233) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-Cytokeratin 2e Antibody [ARC1925] (A309233)

Immunofluorescence analysis of human skin using Anti-Cytokeratin 2e Antibody [ARC1925] (A309233) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).