Mouse monoclonal [C-50] antibody to Cytokeratin 5 + 18.
Specificity
This antibody reacts with Cytokeratin peptides 5 and 18 (58 and 45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Applications
IHC-P, IP, WB, ICC
Dilutions
IHC-P: 10 µg/ml; Positive Control: human prostate.
Reactivity
Rat, Hamster, Porcine, Sheep, Canine, Bovine, Human, Mouse
Cross Reactivity
This antibody does not cross react with Xenopus or Chicken.
Immunogen
Cytoskeleton preparation from HeLa cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
C-50
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
45 kDa, 58 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Figure 2: Western Blot - Anti-Cytokeratin 5 + 18 Antibody [C-50] (A86386)
Western Blotting analysis of whole cell lysate of MCF-7 human breast adenocarcinoma cell line. Lane 1: MW standard. Lane 2: Immunostaining with Anti-Cytokeratin 5 + 18 Antibody (A86386). Lane 3: Immunostaining with Isotype mouse IgG1 control (PPV-06).
Publishing research using Anti-Cytokeratin 5 + 18 Antibody [C-50] (A86386)? Please let us know so that we can list the citation on this page.