Anti-Cytokeratin 5 Antibody [ARC0585] (A80808)

Name: Anti-Cytokeratin 5 Antibody [ARC0585]
Brand: Antibodies.com
SKU: A80808
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0585] antibody to Cytokeratin 5 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Cytokeratin 5 Antibody [ARC0585]

Description

Rabbit monoclonal [ARC0585] antibody to Cytokeratin 5.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 491-590 of human Cytokeratin 5 (KRT5) (P13647).

Sequence

ISVVTSSVSSGYGSGSGYGGGLGGGLGGGLGGGLAGGSSGSYYSSSSGGVGLGGGLSVGGSGFSASSGRGLGVGFGSGGGSSSSVKFVSTTSSSRKSFKS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0585

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

62 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

58 kDa cytokeratin, CK-5, CK5, Cytokeratin-5, Cytokeratin5, DDD, DDD1, EBS2, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, K2C5_HUMAN, K5, Keratin, Keratin 5, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types), keratin complex 2, basic, gene 5, keratin, type II cytoskeletal 5, Keratin-5, Keratin5, KRT 5, Krt5, KRT5A, type II cytoskeletal 5, Type-II keratin Kb5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-Cytokeratin 5 Antibody [ARC0585] (A80808)

Western blot analysis of extracts of A-431 cells, using Anti-Cytokeratin 5 Antibody [ARC0585] (A80808) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-Cytokeratin 5 Antibody [ARC0585] (A80808)

Western blot analysis of extracts of various cell lines, using Anti-Cytokeratin 5 Antibody [ARC0585] (A80808) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-Cytokeratin 5 Antibody [ARC0585] (A80808)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Cytokeratin 5 Antibody [ARC0585] (A80808) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Cytokeratin 5 Antibody [ARC0585] (A80808)

Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using Anti-Cytokeratin 5 Antibody [ARC0585] (A80808) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.