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Anti-Cytokeratin 8 Antibody (A12853)

Western Blot - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.com Western Blot - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.com Immunofluorescence - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Cytokeratin 8 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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orders@antibodies.com

Name
Anti-Cytokeratin 8 Antibody
Description
Rabbit polyclonal antibody to Cytokeratin 8.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-483 of human Cytokeratin 8 (KRT8) (NP_002264.1).
Sequence
MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
54 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CARD2, CK 8, CK-8, CK8, CYK8, CYKER, Cytokeratin endo A, Cytokeratin-8, DreK8, EndoA, K2C8, K2C8_HUMAN, K8, Keratin 8, Keratin type II cytoskeletal 8, Keratin, type II cytoskeletal 8, Keratin-8, KO, Krt 2.8, KRT8, MGC118110, MGC174782, MGC53564, MGC85764, sb:cb186, Type-II keratin Kb8
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Cytokeratin 8 Antibody (A12853)
Western blot analysis of extracts of various cell lines, using Anti-Cytokeratin 8 Antibody (A12853) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Cytokeratin 8 Antibody (A12853)
Western blot analysis of extracts of Rat liver, using Anti-Cytokeratin 8 Antibody (A12853) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cytokeratin 8 Antibody (A12853) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853)
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Cytokeratin 8 Antibody (A12853) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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