Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Dap, DAP 1, DAP-1, DAP1_HUMAN, Death associated protein, Death associated protein 1, Death-associated protein 1, MGC99796, OTTHUMP00000161670, OTTHUMP00000221331
Figure 1: Western Blot - Anti-DAP1 Antibody (A15090)
Western blot analysis of extracts of various cell lines, using Anti-DAP1 Antibody (A15090) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-DAP1 Antibody (A15090) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
