Anti-DARPP32 Antibody (A14080)

Name: Anti-DARPP32 Antibody
Brand: Antibodies.com
SKU: A14080
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to DARPP32 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-DARPP32 Antibody

Description

Rabbit polyclonal antibody to DARPP32.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human DARPP32 (NP_115568.2).

Sequence

MDPKDRKKIQFSVPAPPSQLDPRQVEMIRRRRPTPAMLFRLSEHSSPEEEASPHQRASGEGHHLKSKRPNPCAYTPPSLKAVQRIAESHLQSISNLNENQASEEEDELGELRELGY

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

34 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DARPP 32, DARPP-32, Dopamine and cAMP regulated neuronal phosphoprotein, Dopamine and cAMP regulated neuronal phosphoprotein 32, Dopamine and cAMP regulated phosphoprotein, Dopamine and cAMP regulated phosphoprotein DARPP 32, Dopamine and cAMP regulated phosphoprotein DARPP32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, IPPD, Neuronal phosphoprotein DARPP 32, PPP1R1B, PPR1B_HUMAN, Protein phosphatase 1 regulatory (inhibitor) subunit 1B, Protein phosphatase 1 regulatory inhibitor subunit 1B, Protein phosphatase 1 regulatory subunit 1B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-DARPP32 Antibody (A14080)

Western blot analysis of extracts of various cell lines, using Anti-DARPP32 Antibody (A14080) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunofluorescence - Anti-DARPP32 Antibody (A14080)

Immunofluorescence analysis of SH-SY5Y cells using Anti-DARPP32 Antibody (A14080) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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