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Anti-DARS2 Antibody (A10227)

Western Blot - Anti-DARS2 Antibody (A10227) - Antibodies.com Western Blot - Anti-DARS2 Antibody (A10227) - Antibodies.com Immunohistochemistry - Anti-DARS2 Antibody (A10227) - Antibodies.com Immunohistochemistry - Anti-DARS2 Antibody (A10227) - Antibodies.com Immunohistochemistry - Anti-DARS2 Antibody (A10227) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to DARS2 for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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orders@antibodies.com

Name
Anti-DARS2 Antibody
Description
Rabbit polyclonal antibody to DARS2.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 406-645 of human DARS2 (NP_060592.2).
Sequence
ANRNWNSPVANFIMESQRLELIRLMETQEEDVVLLTAGEHNKACSLLGKLRLECADLLETRGVVLRDPTLFSFLWVVDFPLFLPKEENPRELESAHHPFTAPHPSDIHLLYTEPKKARSQHYDLVLNGNEIGGGSIRIHNAELQRYILATLLKEDVKMLSHLLQALDYGAPPHGGIALGLDRLICLVTGSPSIRDVIAFPKSFRGHDLMSNTPDSVPPEELKPYHIRVSKPTDSKAERAH
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
74 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Aspartate tRNA ligase, Aspartate tRNA ligase 2 mitochondrial, Aspartate tRNA ligase mitochondrial, Aspartate--tRNA ligase, Aspartyl tRNA synthetase 2 (mitochondrial), Aspartyl tRNA synthetase mitochondrial, Aspartyl-tRNA synthetase, AspRS, FLJ10514, LBSL, mitochondrial, MT ASPRS, RP3 383J4.2, SYDM_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-DARS2 Antibody (A10227) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-DARS2 Antibody (A10227)
Western blot analysis of extracts of various cell lines, using Anti-DARS2 Antibody (A10227) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Western Blot - Anti-DARS2 Antibody (A10227) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-DARS2 Antibody (A10227)
Western blot analysis of extracts of various cell lines, using Anti-DARS2 Antibody (A10227) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunohistochemistry - Anti-DARS2 Antibody (A10227) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-DARS2 Antibody (A10227)
Immunohistochemistry analysis of paraffin-embedded human liver damage using Anti-DARS2 Antibody (A10227) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-DARS2 Antibody (A10227) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-DARS2 Antibody (A10227)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-DARS2 Antibody (A10227) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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