Anti-DCP2 Antibody [PCRP-DCP2-1D6] (A277575)

Mouse monoclonal [PCRP-DCP2-1D6] antibody to DCP2 for IP, Flow Cytometry and IF.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-DCP2 Antibody [PCRP-DCP2-1D6]
Description
Mouse monoclonal [PCRP-DCP2-1D6] antibody to DCP2.
Specificity
The major pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5' to 3' exonucleolytic degradation. Human decapping enzyme 2 (hDcp2) is an mRNA decapping enzyme which contains intrinsic decapping activity. In nonsense-mediated decay, the human decapping complex, made up of hDcp1 and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. The decapping activator complex (Lsm1p-7p) is also involved in the recruitment of the decapping complex, indicated by data showing that Lsm1p-7p enhances the co-immunoprecipitation of the complex with mRNA. Dcp2 specifically hydrolyzes methylated capped RNA to release m7GDP, thereby aiding in mRNA degradation. Both Dcp1 and Dcp2 co-localize in the cytoplasm, which is consistent with their role in mRNA decay
Applications
IP, Flow Cytometry, IF
Dilutions
IP: 1-2µg / 100-500µg proteins, Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml
Reactivity
Human
Cross Reactivity
This antibody is predicted to cross react with Mouse and Rat.
Immunogen
Recombinant full-length human DCP2 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
PCRP-DCP2-1D6
Isotype
IgG2a
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
48.4 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-DCP2 Antibody [PCRP-DCP2-1D6] - BSA and Azide free (A278163).
Synonyms
DCP2 decapping enzyme homolog (S. cerevisiae), DCP2, S. cerevisiae, homolog of, DCP2_HUMAN, decapping enzyme 2, S. cerevisiae, homolog of, decapping enzyme homolog (S. cerevisiae), FLJ33245, hDpc, m7GpppN-mRNA hydrolase, mRNA decapping enzyme 2, mRNA-decapping enzyme 2, Nucleoside diphosphate-linked moiety X motif 20, nudix (nucleoside diphosphate linked moiety X)-type motif 20, Nudix motif 20, NUDT20
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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