Anti-DDIT3 Antibody [ARC51429] (A306793)

Name: Anti-DDIT3 Antibody [ARC51429]
Brand: Antibodies.com
SKU: A306793

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This product is discontinued

Anti-DDIT3 Antibody [ARC51429] (A306793) has been discontinued and is no longer available.

View all Anti-DDIT3 Antibodies.

Specifications

Name

Anti-DDIT3 Antibody [ARC51429]

Description

Rabbit monoclonal [ARC51429] antibody to DDIT3.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DDIT3/CHOP (NP_004074.2).

Sequence

MAAESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEEESKIFTTLDPASLAWLTEEEPEPAEVTSTSQSPHSPDSSQSSLAQEEEEEDQG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51429

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

27 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

C/EBP homologous protein, C/EBP Homology Protein, C/EBP zeta, C/EBP-homologous protein, C/EBP-homologous protein 10, CCAAT/enhancer binding protein homologous protein, CEBPZ, CHOP, CHOP 10, CHOP-10, CHOP10, DDIT 3, DDIT-3, DDIT3_HUMAN, DNA Damage Inducible Transcript 3, DNA damage-inducible transcript 3 protein, GADD 153, GADD153, Growth Arrest and DNA Damage Inducible Protein 153, Growth arrest and DNA damage inducible protein GADD153, Growth arrest and DNA damage-inducible protein GADD153, MGC4154

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-DDIT3 Antibody [ARC51429] (A306793)

Western blot analysis of extracts of various cell lines, using Anti-DDIT3 Antibody [ARC51429] (A306793) at 1:1,000 dilution. C2C12 and C6 cells were treated by tunicamycin (2 µg/ml) for 8 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-DDIT3 Antibody [ARC51429] (A306793)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-DDIT3 Antibody [ARC51429] (A306793) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-DDIT3 Antibody [ARC51429] (A306793)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-DDIT3 Antibody [ARC51429] (A306793) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-DDIT3 Antibody [ARC51429] (A306793)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-DDIT3 Antibody [ARC51429] (A306793) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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