Anti-DDX5 Antibody [ARC0575] (A80579)

Name: Anti-DDX5 Antibody [ARC0575]
Brand: Antibodies.com
SKU: A80579

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This product is discontinued

Anti-DDX5 Antibody [ARC0575] (A80579) has been discontinued and is no longer available.

View all Anti-DDX5 Antibodies.

Specifications

Name

Anti-DDX5 Antibody [ARC0575]

Description

Rabbit monoclonal [ARC0575] antibody to DDX5.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DDX5 (P17844).

Sequence

MSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEAN

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0575

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

69 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ATP dependent RNA helicase DDX5, DDX 5, DDX5_HUMAN, DEAD (Asp Glu Ala Asp) box helicase 5, DEAD (Asp Glu Ala Asp) box polypeptide 5, DEAD box 5, DEAD box protein 5, DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD), G17P1, HELR, HLR1, HUMP68, P68, p68 RNA helicase, Probable ATP dependent RNA helicase DDX5, Probable ATP-dependent RNA helicase DDX5, RNA helicase p68

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-DDX5 Antibody [ARC0575] (A80579)

Western blot analysis of extracts of various cell lines, using Anti-DDX5 Antibody [ARC0575] (A80579) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-DDX5 Antibody [ARC0575] (A80579)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-DDX5 Antibody [ARC0575] (A80579) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-DDX5 Antibody [ARC0575] (A80579)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-DDX5 Antibody [ARC0575] (A80579) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-DDX5 Antibody [ARC0575] (A80579)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-DDX5 Antibody [ARC0575] (A80579) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.