Anti-DDX6 Antibody (A295541)

Name: Anti-DDX6 Antibody
Brand: Antibodies.com
SKU: A295541
Price: 425.0 USD
Availability: InStock

This product is identical to Novus (NB200-191). Discover more for less.

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$425

Rabbit polyclonal antibody to DDX6 for WB, IP and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-DDX6 Antibody

Description

Rabbit polyclonal antibody to DDX6.

Applications

WB, IP, IHC

Dilutions

IHC: 1:100-1:500, IP: 2-5 µg / 1 mg lysate, WB: 1:5,000-1:15,000

Reactivity

Human, Mouse

Immunogen

Synthetic peptide within amino acids 1-50 of human DDX6.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Antigen affinity purification.

Concentration

200 µg/ml

Product Form

Liquid

Formulation

Supplied in Tris-Buffered Saline with 0.1% BSA and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.

Synonyms

ATP dependent RNA helicase DDX6, ATP dependent RNA helicase p54, DEAD (Asp Glu Ala Asp) box polypeptide 6, DEAD box 6, DEAD box protein 6, DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RNA helicase 54kD), FLJ36338, HLR2, Oncogene RCK, P54, Probable ATP-dependent RNA helicase DDX6, RCK

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Immunohistochemistry - Anti-DDX6 Antibody (A295541)

Sample: FFPE section of human prostate carcinoma. Antibody: Anti-DDX6 Antibody (A295541) was used at a dilution of 1:200 (1 µg/ml). Detection: DAB.

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Figure 2: Immunohistochemistry - Anti-DDX6 Antibody (A295541)

Sample: FFPE section of mouse renal cell carcinoma. Antibody: Anti-DDX6 Antibody (A295541) was used at a dilution of 1:200 (1 µg/ml). Detection: DAB.

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Figure 3: Western Blot - Anti-DDX6 Antibody (A295541)

Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, MCF-7, Jurkat, and HepG2 cells prepared using NETN lysis buffer. Antibody: Anti-DDX6 Antibody (A295541) was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Figure 4: Immunoprecipitation - Anti-DDX6 Antibody (A295541)

Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Anti-DDX6 Antibody (A295541) was used for IP at 6 µg per reaction. DDX6 was also immunoprecipitated using a previous batch of this antibody and a second antibody against a different epitope of DDX6. For blotting immunoprecipitated DDX6, Anti-DDX6 Antibody (A295541) was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.