Anti-DENN Antibody [ARC2860] (A309107)

Name: Anti-DENN Antibody [ARC2860]
Brand: Antibodies.com
SKU: A309107

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This product is discontinued

Anti-DENN Antibody [ARC2860] (A309107) has been discontinued and is no longer available.

View all Anti-DENN Antibodies.

Specifications

Name

Anti-DENN Antibody [ARC2860]

Description

Rabbit monoclonal [ARC2860] antibody to DENN.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1548-1647 of human DENN (Q8WXG6).

Sequence

VQDLKTGEGGLLQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPPRPVSS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2860

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

220 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Differentially expressed in normal and neoplastic cells, FLJ35600, IG20, Insulinoma glucagonoma clone 20, KIAA0358, MADD, MADD_HUMAN, MAP kinase-activating death domain protein, Rab3 GDP/GTP exchange factor, RAB3GEP

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-DENN Antibody [ARC2860] (A309107)

Western blot analysis of extracts of various cell lines, using Anti-DENN Antibody [ARC2860] (A309107) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-DENN Antibody [ARC2860] (A309107)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-DENN Antibody [ARC2860] (A309107) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-DENN Antibody [ARC2860] (A309107)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-DENN Antibody [ARC2860] (A309107) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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