Anti-DGCR8 Antibody (A305592)

Name: Anti-DGCR8 Antibody
Brand: Antibodies.com
SKU: A305592
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to DGCR8 for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-DGCR8 Antibody

Description

Rabbit polyclonal antibody to DGCR8.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 180-340 of human DGCR8 (NP_073557.3).

Sequence

DKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGAGGFTAKAIVQRDRVDEEALNFPYEDDFDNDVDALLEEGLCAPKKRRTEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSI

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

110 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

C22orf12, D16H22S788E, D16Wis2, DGCR 8, DGCR8 microprocessor complex subunit, DGCR8_HUMAN, DGCRK 6, DGCRK6, DiGeorge syndrome critical region 8, DiGeorge syndrome critical region gene 8, Gy1, Microprocessor complex subunit DGCR8, pasha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-DGCR8 Antibody (A305592)

Western blot analysis of extracts of various cell lines, using Anti-DGCR8 Antibody (A305592) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-DGCR8 Antibody (A305592)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-DGCR8 Antibody (A305592) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-DGCR8 Antibody (A305592)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-DGCR8 Antibody (A305592) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-DGCR8 Antibody (A305592)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-DGCR8 Antibody (A305592) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.