Anti-DIS Antibody (A15179)

Name: Anti-DIS Antibody
Brand: Antibodies.com
SKU: A15179
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to DIS for WB and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-DIS Antibody

Description

Rabbit polyclonal antibody to DIS.

Applications

WB, IP

Dilutions

WB: 1:500-1:1,000, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CCAR1 (NP_060707.2).

Sequence

MAQFGGQKNPPWATQFTATAVSQPAALGVQQPSLLGASPTIYTQQTALAAAGLTTQTPANYQLTQTAALQQQAAAAAAALQQQYSQPQQALYSVQQQLQQPQQTLLTQPAVALPTSLSLSTPQPTAQITVSYPTPRSSQQQTQPQKQRVFTGVVTKLHDTFGFVDEDVFFQLSAVKGKTPQVGDRVLVEATYNPNMPFKW

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

132 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CARP-1, Carp1, Ccar1, CCAR1_HUMAN, Cell cycle and apoptosis regulatory protein 1, cell division cycle and apoptosis regulator 1, Cell division cycle and apoptosis regulator protein 1, Death inducer with SAP domain, FLJ10590, FLJ10839, FLJ13376, FLJ20734, MGC7730, RP11-437A18.1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-DIS Antibody (A15179)

Western blot analysis of extracts of Mouse testis, using Anti-DIS Antibody (A15179) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 5s.

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Figure 2: Western Blot - Anti-DIS Antibody (A15179)

Western blot analysis of extracts of HepG2 cells, using Anti-DIS Antibody (A15179) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-DIS Antibody (A15179)

Western blot analysis of extracts of Rat spleen, using Anti-DIS Antibody (A15179) at 1:700 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.

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Figure 4: Western Blot - Anti-DIS Antibody (A15179)

Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-DIS Antibody (A15179). This Western blot was performed on the immunoprecipitate using Anti-DIS Antibody (A15179) at a dilution of 1:500.

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