Anti-Dishevelled 3 Antibody (A11691)

Name: Anti-Dishevelled 3 Antibody
Brand: Antibodies.com
SKU: A11691
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Dishevelled 3 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Dishevelled 3 Antibody

Description

Rabbit polyclonal antibody to Dishevelled 3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:20-1:50

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 580-650 of human DVL3 (NP_004414.3).

Sequence

RSGSDRRKEKDPKAGDSKSGGSGSESDHTTRSSLRGPRERAPSERSGPAASEHSHRSHHSLASSLRSHHTH

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

93 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Dishevelled 3 (homologous to Drosophila dsh), Dishevelled 3, dsh homolog (Drosophila), Dishevelled, dsh homolog 3 (Drosophila), Dishevelled-3, DSH homolog 3, dvl3, DVL3_HUMAN, KIAA0208, OTTHUMP00000210777, OTTHUMP00000210778, Segment polarity protein dishevelled homolog DVL 3, Segment polarity protein dishevelled homolog DVL-3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Dishevelled 3 Antibody (A11691)

Western blot analysis of extracts of various cell lines, using Anti-Dishevelled 3 Antibody (A11691) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-Dishevelled 3 Antibody (A11691)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Dishevelled 3 Antibody (A11691) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Dishevelled 3 Antibody (A11691)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Dishevelled 3 Antibody (A11691) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Dishevelled 3 Antibody (A11691)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Dishevelled 3 Antibody (A11691) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.