Anti-DNA PKcs Antibody [ARC51265] (A308615)

Name: Anti-DNA PKcs Antibody [ARC51265]
Brand: Antibodies.com
SKU: A308615

Enlarge Image

This product is discontinued

Anti-DNA PKcs Antibody [ARC51265] (A308615) has been discontinued and is no longer available.

View all Anti-DNA PKcs Antibodies.

Specifications

Name

Anti-DNA PKcs Antibody [ARC51265]

Description

Rabbit monoclonal [ARC51265] antibody to DNA PKcs.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 3993-4128 of human DNA-PKcs (NP_008835.5).

Sequence

SDPGLLTNTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNILGRTWEGWEPWM

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51265

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

469 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DNA dependent protein kinase catalytic subunit, DNA PK catalytic subunit, DNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, DNA-PKcs, DNAPK, DNAPK catalytic subunit, DNPK 1, DNPK1, Hyper radiosensitivity of murine scid mutation, complementing 1, Hyperradiosensitivity complementing 1, mouse, homolog of 1, HYRC, HYRC 1, HYRC1, IMD26, p350, p460, PKRDC, PRKDC, PRKDC_HUMAN, Protein Kinase DNA Activated Catalytic Polypeptide, XRCC 7, XRCC7

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

Enlarge Image

Figure 1: Western Blot - Anti-DNA PKcs Antibody [ARC51265] (A308615)

Western blot analysis of various lysates, using Anti-DNA PKcs Antibody [ARC51265] (A308615) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-DNA PKcs Antibody [ARC51265] (A308615)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-DNA PKcs Antibody [ARC51265] (A308615) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-DNA PKcs Antibody [ARC51265] (A308615)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-DNA PKcs Antibody [ARC51265] (A308615) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunofluorescence - Anti-DNA PKcs Antibody [ARC51265] (A308615)

Immunofluorescence analysis of HeLa using Anti-DNA PKcs Antibody [ARC51265] (A308615) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Publishing research using Anti-DNA PKcs Antibody [ARC51265] (A308615)? Please let us know so that we can list the citation on this page.