Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DNA directed DNA polymerase beta, DNA pol beta, DNA polymerase beta subunit, DPOLB_HUMAN, MGC125976, Pol B, Pol beta, POLB, Polymerase (DNA directed) beta
Figure 1: Western Blot - Anti-DNA Polymerase beta Antibody [ARC0752] (A307402)
Western blot analysis of extracts of various cell lines, using Anti-DNA Polymerase beta Antibody [ARC0752] (A307402) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-DNA Polymerase beta Antibody [ARC0752] (A307402) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-DNA Polymerase beta Antibody [ARC0752] (A307402) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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