Anti-Dnmt1 Antibody [ARC51348] (A307587)

Name: Anti-Dnmt1 Antibody [ARC51348]
Brand: Antibodies.com
SKU: A307587
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC51348] antibody to Dnmt1 for WB, ICC/IF, IP and ChIP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Dnmt1 Antibody [ARC51348]

Description

Rabbit monoclonal [ARC51348] antibody to Dnmt1.

Applications

WB, ICC/IF, IP, ChIP

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNMT1 (NP_001370.1).

Sequence

MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYN

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51348

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

200 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADCADN, AIM, CXXC finger protein 9, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine 5 ) methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase 1, DNA methyltransferase HsaI, DNA methyltransferase M.HsaI., DNA MTase, DNA MTase HsaI, DNMT, DNMT 1, Dnmt1o, DNMT1_HUMAN, FLJ16293, HSN1E, M.HsaI, MCMT, Met1, MGC104992, mMmul, MommeD2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Dnmt1 Antibody [ARC51348] (A307587)

Western blot analysis of extracts of various cell lines, using Anti-Dnmt1 Antibody [ARC51348] (A307587) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Western Blot - Anti-Dnmt1 Antibody [ARC51348] (A307587)

Western blot analysis of extracts from wild type (WT) and DNMT1 knockdown (KD) 293T cells, using Anti-Dnmt1 Antibody [ARC51348] (A307587) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunofluorescence - Anti-Dnmt1 Antibody [ARC51348] (A307587)

Immunofluorescence analysis of C6 cells using Anti-Dnmt1 Antibody [ARC51348] (A307587). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-Dnmt1 Antibody [ARC51348] (A307587)

Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-Dnmt1 Antibody [ARC51348] (A307587). This Western blot was performed on the immunoprecipitate using Anti-Dnmt1 Antibody [ARC51348] (A307587) at a dilution of 1:1000.

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