Anti-Dnmt3b Antibody (A11580)

Name: Anti-Dnmt3b Antibody
Brand: Antibodies.com
SKU: A11580
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Dnmt3b for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Dnmt3b Antibody

Description

Rabbit polyclonal antibody to Dnmt3b.

Applications

WB, ICC/IF

Dilutions

WB: 1:200-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 345-475 of human DNMT3B (Q9UBC3).

Sequence

IEGLKPNNTQPVVNKSKVRRAGSRKLESRKYENKTRRRTADDSATSDYCPAPKRLKTNCYNNGKDRGDEDQSREQMASDVANNKSSLEDGCLSCGRKNPVSFHPLFEGGLCQTCRDRFLELFYMYDDDGYQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

110 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cytosine 5methyltransferase 3B, DNA, DNA (cytosine 5) methyltransferase 3 beta, DNA (cytosine 5)-methyltransferase 3B, DNA (cytosine-5)-methyltransferase 3B, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, DNM3B_HUMAN, EC 2.1.1.37, ICF, ICF1, M.HsaIIIB, MGC124407, RP23-89H14.3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Dnmt3b Antibody (A11580)

Western blot analysis of extracts of various cell lines, using Anti-Dnmt3b Antibody (A11580) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunofluorescence - Anti-Dnmt3b Antibody (A11580)

Immunofluorescence analysis of C6 cells using Anti-Dnmt3b Antibody (A11580) at dilution of 100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Dnmt3b Antibody (A11580)

Immunofluorescence analysis of HeLa cells using Anti-Dnmt3b Antibody (A11580) at dilution of 100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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