Anti-Doublecortin Antibody (A92280)

Name: Anti-Doublecortin Antibody
Brand: Antibodies.com
SKU: A92280

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This product is discontinued

Anti-Doublecortin Antibody (A92280) has been discontinued and is no longer available.

View all Anti-Doublecortin Antibodies.

Specifications

Name

Anti-Doublecortin Antibody

Description

Rabbit polyclonal antibody to Doublecortin.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 265-365 of human Doublecortin (NP_835365.1).

Sequence

SLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSGNDQDANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

45 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DBCN, Dbct, DC, DCX, DCX_HUMAN, Doublecortex, Doublin, FLJ51296, Lis X, Lis-X, Lissencephalin X, Lissencephalin-X, Lissencephaly X linked, Lissencephaly X linked doublecortin, LISX, Neuronal migration protein doublecortin, OTTHUMP00000023859, OTTHUMP00000023860, OTTHUMP00000216315, OTTHUMP00000216316, SCLH, XLIS

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Doublecortin Antibody (A92280)

Western blot analysis of extracts of various cell lines, using Anti-Doublecortin Antibody (A92280) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Immunofluorescence - Anti-Doublecortin Antibody (A92280)

Immunofluorescence analysis of Neuro-2a cells using Anti-Doublecortin Antibody (A92280) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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