Anti-DYKDDDDK Tag Antibody (A283025)

Anti-DYKDDDDK Tag Antibody

Rabbit polyclonal antibody to DYKDDDDK Tag.

This antibody recognises the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins.Rabbit anti DYKDDDDK Tag antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays.In Western blotting of bacterial extracts Rabbit anti DYKDDDDK Tag antibody does not cross-react with any endogenous proteins.

WB, ELISA

ELISA: 1:9,000 - 1:450,000, WB: 1:2,000 - 1:10,000

Species Independent

Synthetic peptide sequence DYKDDDDK corresponding to the enterokinase cleavage site, conjugated to Keyhole Limpet Haemocyanin. Residues of cysteine and glycine were used to facilitate coupling at the C-terminus end.

DYKDDDDK

Rabbit

Polyclonal

IgG

500 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti DYKDDDDK Tag antibody recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins.Rabbit anti DYKDDDDK Tag antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays.In Western blotting of bacterial extracts Rabbit anti DYKDDDDK Tag antibody does not cross-react with any endogenous proteins.

DDDDK epitope tag, DDDDK tag, DDDK, ddk, DYKDDDDK, DYKDDDDK epitope tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG, FLAG tag