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Anti-E Cadherin Antibody [ARC51012] (A308267)

Western Blot - Anti-E Cadherin Antibody [ARC51012] (A308267) - Antibodies.com Western Blot - Anti-E Cadherin Antibody [ARC51012] (A308267) - Antibodies.com Immunohistochemistry - Anti-E Cadherin Antibody [ARC51012] (A308267) - Antibodies.com Immunohistochemistry - Anti-E Cadherin Antibody [ARC51012] (A308267) - Antibodies.com Immunofluorescence - Anti-E Cadherin Antibody [ARC51012] (A308267) - Antibodies.com
This product is discontinued

Anti-E Cadherin Antibody [ARC51012] (A308267) has been discontinued and is no longer available.

View all Anti-E Cadherin Antibodies.

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Western Blot - Anti-E Cadherin Antibody (A83532)
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Anti-E Cadherin Antibody [67A4]
Mouse monoclonal [67A4] antibody to E Cadherin for Flow Cytometry, IP, WB, IHC-Fr and ICC.
Western Blot - Anti-E Cadherin Antibody (A83532)
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Goat polyclonal antibody to E Cadherin for ELISA, WB and IHC.
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1 Citation
Anti-E Cadherin Antibody [67A4]
Mouse monoclonal [67A4] antibody to E Cadherin for Flow Cytometry, IP, WB, IHC-Fr and ICC.
Name
Anti-E Cadherin Antibody [ARC51012]
Description
Rabbit monoclonal [ARC51012] antibody to E Cadherin.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 700-800 of human E-Cadherin (NP_004351.1).
Sequence
PVEAGLQIPAILGILGGILALLILILLLLLFLRRRAVVKEPLLPPEDDTRDNVYYYDEEGGGEEDQDFDLSQLHRGLDARPEVTRNDVAPTLMSVPRYLPR
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC51012
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
135 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Arc 1, CADH1_HUMAN, Cadherin 1, cadherin 1 type 1 E-cadherin, Cadherin1, CAM 120/80, CD 234, CD 324, CD324, CD324 antigen, CDH1, CDHE, E-Cad/CTF3, E-cadherin, ECAD , Epithelial cadherin, epithelial calcium dependant adhesion protein, LCAM, Liver cell adhesion molecule, UVO, Uvomorulin
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-E Cadherin Antibody [ARC51012] (A308267) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-E Cadherin Antibody [ARC51012] (A308267)
Western blot analysis of extracts of various cell lines, using Anti-E Cadherin Antibody [ARC51012] (A308267) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-E Cadherin Antibody [ARC51012] (A308267) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-E Cadherin Antibody [ARC51012] (A308267)
Western blot analysis of extracts of Rat kidney, using Anti-E Cadherin Antibody [ARC51012] (A308267) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry - Anti-E Cadherin Antibody [ARC51012] (A308267) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-E Cadherin Antibody [ARC51012] (A308267)
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-E Cadherin Antibody [ARC51012] (A308267) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-E Cadherin Antibody [ARC51012] (A308267) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-E Cadherin Antibody [ARC51012] (A308267)
Immunohistochemistry analysis of paraffin-embedded mouse Intestine using Anti-E Cadherin Antibody [ARC51012] (A308267) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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