Anti-EAAT1 Antibody [ARC1714] (A306133)

Name: Anti-EAAT1 Antibody [ARC1714]
Brand: Antibodies.com
SKU: A306133
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1714] antibody to EAAT1 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Astrocyte Marker Radial Glia Marker

Specifications

Name

Anti-EAAT1 Antibody [ARC1714]

Description

Rabbit monoclonal [ARC1714] antibody to EAAT1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human EAAT1/SLC1A3 (P43003).

Sequence

GTKENMHREGKIVRVTAADAFLDLIRNMFPPNLVEACFKQFKTNYEKRSFKVPIQANETLVGAVINNVSEAMETLTRITEELVPVPGSVNGVNALGLVVFS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1714

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 - 55 kDa / 90 - 150 kDa / 58 kDa / 130 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EA6, EAA1_HUMAN, Excitatory amino acid transporter 1, FLJ25094, GLAST, GLAST-1, GLAST1, Glial high affinity glutamate transporter, glutamate/aspartate transporter, high affinity, sodium-dependent, High affinity neuronal glutamate transporter, Slc1a3, Sodium dependent glutamate/aspartate transporter, Sodium-dependent glutamate/aspartate transporter 1, Solute carrier family 1 (glial high affinity glutamate transporter) member 3, Solute carrier family 1 member 3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-EAAT1 Antibody [ARC1714] (A306133)

Western blot analysis of extracts of various cell lines, using Anti-EAAT1 Antibody [ARC1714] (A306133) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 2: Western Blot - Anti-EAAT1 Antibody [ARC1714] (A306133)

Western blot analysis of extracts of Rat brain, using Anti-EAAT1 Antibody [ARC1714] (A306133) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 3: Immunohistochemistry - Anti-EAAT1 Antibody [ARC1714] (A306133)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-EAAT1 Antibody [ARC1714] (A306133) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-EAAT1 Antibody [ARC1714] (A306133)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-EAAT1 Antibody [ARC1714] (A306133) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.