Anti-ECE1 Antibody (A14875)

Name: Anti-ECE1 Antibody
Brand: Antibodies.com
SKU: A14875

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This product is discontinued

Anti-ECE1 Antibody (A14875) has been discontinued and is no longer available.

View all Anti-ECE1 Antibodies.

Specifications

Name

Anti-ECE1 Antibody

Description

Rabbit polyclonal antibody to ECE1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 670-770 of human ECE1 (NP_001388.1).

Sequence

ADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

130 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ECE, ECE 1, ECE-1, ECE1_HUMAN, Endothelin converting enzyme 1, Endothelin-converting enzyme 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (1)

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Figure 1: Western Blot - Anti-ECE1 Antibody (A14875)

Western blot analysis of extracts from normal (control) and ECE1 knockout (KO) HeLa cells, using Anti-ECE1 Antibody (A14875) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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