Anti-EED Antibody (A14710)

Name: Anti-EED Antibody
Brand: Antibodies.com
SKU: A14710
Price: 335.0 USD
Availability: InStock

Enlarge Image

$335

Rabbit polyclonal antibody to EED for WB, ICC/IF and IP.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-EED Antibody

Description

Rabbit polyclonal antibody to EED.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IP: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-400 of human EED (NP_694536.1).

Sequence

MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 - 70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EED protein, EED, mouse, homolog of, EED_HUMAN, Embryonic ectoderm development, Embryonic ectoderm development isoform a, Embryonic ECTODERM development protein homolog, Embryonic ectoderm development protein, mouse, homolog of, hEED, OTTHUMP00000235483, OTTHUMP00000235484, OTTHUMP00000235485, Polycomb protein eed, WAIT 1, WAIT-1, WAIT1, WD protein associating with integrin cytoplasmic tails 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-EED Antibody (A14710)

Western blot analysis of extracts of various cell lines, using Anti-EED Antibody (A14710) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Enlarge Image

Figure 2: Immunofluorescence - Anti-EED Antibody (A14710)

Immunofluorescence analysis of A-549 cells using Anti-EED Antibody (A14710) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Figure 3: Immunofluorescence - Anti-EED Antibody (A14710)

Immunofluorescence analysis of C6 cells using Anti-EED Antibody (A14710) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Figure 4: Immunofluorescence - Anti-EED Antibody (A14710)

Immunofluorescence analysis of NIH/3T3 cells using Anti-EED Antibody (A14710) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).