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Anti-EF-Ts Antibody (A89421)

Western Blot - Anti-EF-Ts Antibody (A89421) - Antibodies.com Immunofluorescence - Anti-EF-Ts Antibody (A89421) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to EF-Ts for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-EF-Ts Antibody
Description
Rabbit polyclonal antibody to EF-Ts.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 245-346 of human TSFM (NP_001166167).
Sequence
PSLHKLVLGKYGALVICETSEQKTNLEDVGRRLGQHVVGMAPLSVGSLDDEPGGEAETKMLSQPYLLDPSITLGQYVQPQGVSVVDFVRFECGEGEEAAETE
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
35 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
COXPD 3, COXPD3, EF TS, EF Tsmt, EF-TsMt, EFTS, EFTSMT, EFTS_HUMAN, Elongation factor Ts, Elongation factor Ts mitochondrial, HGNC12367, mitochondrial, Mitochondrial elongation factor Ts, Ts translation elongation factor mitochondrial, TSFM, TSMT
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-EF-Ts Antibody (A89421) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-EF-Ts Antibody (A89421)
Western blot analysis of extracts of various cell lines, using Anti-EF-Ts Antibody (A89421) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence - Anti-EF-Ts Antibody (A89421) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-EF-Ts Antibody (A89421)
Immunofluorescence analysis of U-2 OS cells using Anti-EF-Ts Antibody (A89421) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-EF-Ts Antibody (A89421)? Please let us know so that we can list the citation on this page.
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