The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu, which agrees with the sequence of native EGF human.N-terminal methionine has been completely removed enzymatically.
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Product Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Each lyophilized mg contains 0.15M NaCl, 0.025M sodium bicarbonate, pH-7.5.
Storage
Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
