Rabbit monoclonal [ARC53785] antibody to eIF4EBP1 (phospho Thr70).
Applications
WB
Dilutions
WB: 1:2,000-1:6,000
Reactivity
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around T70 of human Phospho-eIF4EBP1-T70 (NP_004086.1).
Sequence
TKTPP
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC53785
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity purification.
Molecular Weight
15 - 20 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
4E-BP1, 4EBP1, 4EBP1_HUMAN, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Figure 1: Western Blot - Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785] (A308921)
Western blot analysis of extracts of various cell lines, using Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785] (A308921) at 1:5,000 dilution. 293T cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Figure 2: Western Blot - Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785] (A308921)
Western blot analysis of extracts of NIH/3T3, using Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785] (A308921) at 1:5,000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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