Anti-Elastin Antibody [ARC58662] (A310009)

Name: Anti-Elastin Antibody [ARC58662]
Brand: Antibodies.com
SKU: A310009

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This product is discontinued

Anti-Elastin Antibody [ARC58662] (A310009) has been discontinued and is no longer available.

View all Anti-Elastin Antibodies.

Specifications

Name

Anti-Elastin Antibody [ARC58662]

Description

Rabbit monoclonal [ARC58662] antibody to Elastin.

Applications

WB, IHC

Dilutions

WB: 1:2,000-1:7,000, IHC: 1:500-1:1,000

Reactivity

Human

Immunogen

Recombinant protein of human ELN.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC58662

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

68 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ELN, ELN_HUMAN, SVAS, Tropoelastin, WBS, WS

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Elastin Antibody [ARC58662] (A310009)

Western blot analysis of various lysates, using Anti-Elastin Antibody [ARC58662] (A310009) at 1:6,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-Elastin Antibody [ARC58662] (A310009)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Elastin Antibody [ARC58662] (A310009) at a dilution of 1:600 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Elastin Antibody [ARC58662] (A310009)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Elastin Antibody [ARC58662] (A310009) at a dilution of 1:600 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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