Anti-HuR / ELAVL1 Antibody (A13545)

Name: Anti-HuR / ELAVL1 Antibody
Brand: Antibodies.com
SKU: A13545
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to HuR / ELAVL1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-HuR / ELAVL1 Antibody

Description

Rabbit polyclonal antibody to HuR / ELAVL1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:100-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human HuR/ELAVL1 (NP_001410.2).

Sequence

MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

37 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ELAV (embryonic lethal abnormal vision Drosophila) like 1, ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R), ELAV like 1, ELAV like RNA binding protein 1, ELAV-like protein 1, ELAV1, ELAV1_HUMAN, Elavl1, Embryonic lethal abnormal vision drosophila homolog like 1, Hu Antigen R, Hu-antigen R, Hua, HuR, MelG

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-HuR / ELAVL1 Antibody (A13545)

Western blot analysis of extracts of various cell lines, using Anti-HuR / ELAVL1 Antibody (A13545) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.

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Figure 2: Western Blot - Anti-HuR / ELAVL1 Antibody (A13545)

Western blot analysis of extracts from normal (control) and HuR/ELAVL1 knockout (KO) 293T cells, using Anti-HuR / ELAVL1 Antibody (A13545) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-HuR / ELAVL1 Antibody (A13545)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-HuR / ELAVL1 Antibody (A13545) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-HuR / ELAVL1 Antibody (A13545)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-HuR / ELAVL1 Antibody (A13545) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.