Anti-Emerin Antibody [ARC0921] (A308435)

Name: Anti-Emerin Antibody [ARC0921]
Brand: Antibodies.com
SKU: A308435
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0921] antibody to Emerin for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Emerin Antibody [ARC0921]

Description

Rabbit monoclonal [ARC0921] antibody to Emerin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-254 of human Emerin/EMD (P50402).

Sequence

RERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQDRQVPLWGQLLLFLVFVIVLFFIYHFMQAEEGNPF

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0921

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

30 kDa / 35 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EDMD, Emd, EMD_HUMAN, Emery Dreifuss muscular dystrophy, STA

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Emerin Antibody [ARC0921] (A308435)

Western blot analysis of extracts of various cell lines, using Anti-Emerin Antibody [ARC0921] (A308435) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-Emerin Antibody [ARC0921] (A308435)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Emerin Antibody [ARC0921] (A308435) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Emerin Antibody [ARC0921] (A308435)

Confocal imaging of HeLa cells using Anti-Emerin Antibody [ARC0921] (A308435), at a dilution of 1:100, (red). The cells were counterstained with Anti-alpha Tubulin Antibody, at a dilution of 1:100, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.

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