Mouse monoclonal [NP4D6] antibody to ENPP3/B10 (PE).
Specificity
This antibody reacts with an extracellular epitope of CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation.
Applications
Flow Cytometry, ICC
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests; Extracellular and Intracellular staining.
Reactivity
Human
Immunogen
HEK-293 cells transfected with human CD203c.
Host
Mouse
Clonality
Monoclonal
Clone ID
NP4D6
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Surface staining of activated human basophil by Anti-CD63 Antibody [MEM-259] (green) and Anti-CD203c Antibody [NP4D6] (red); merged signal yellow. DNA visualized by DAPI (blue).
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![Flow Cytometry - Anti-CD203c Antibody [NP4D6] (PE) (A86534) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86534_707.jpg?profile=product_top)
![Immunocytochemistry - Anti-CD203c Antibody [NP4D6] (PE) (A86533) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86534_708.jpg?profile=product_top)
![Flow Cytometry - Anti-CD203c Antibody [NP4D6] (PE) (A86534) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86534_707.jpg?profile=product_top_thumb)
![Immunocytochemistry - Anti-CD203c Antibody [NP4D6] (PE) (A86533) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86534_708.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD203c Antibody [NP4D6] (PE) (A86534) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86534_707.jpg?profile=product_image)
![Immunocytochemistry - Anti-CD203c Antibody [NP4D6] (PE) (A86533) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86534_708.jpg?profile=product_image)