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Anti-ENPP5 Antibody [ARC2780] (A305501)

Western Blot - Anti-ENPP5 Antibody [ARC2780] (A305501) - Antibodies.com Western Blot - Anti-ENPP5 Antibody [ARC2780] (A305501) - Antibodies.com Immunofluorescence - Anti-ENPP5 Antibody [ARC2780] (A305501) - Antibodies.com Immunofluorescence - Anti-ENPP5 Antibody [ARC2780] (A305501) - Antibodies.com
This product is discontinued

Anti-ENPP5 Antibody [ARC2780] (A305501) has been discontinued and is no longer available.

View all Anti-ENPP5 Antibodies.

Name
Anti-ENPP5 Antibody [ARC2780]
Description
Rabbit monoclonal [ARC2780] antibody to ENPP5.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 378-477 of human ENPP5 (Q9UJA9).
Sequence
CHLLNITAMPHNGSFWNVQDLLNSAMPRVVPYTQSTILLPGSVKPAEYDQEGSYPYFIGVSLGSIIVIVFFVIFIKHLIHSQIPALQDMHAEIAQPLLQA
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2780
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
54 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
E-NPP 5, E-NPP5, Ectonucleotide pyrophosphatase/phosphodiesterase 5, ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative function), Ectonucleotide pyrophosphatase/phosphodiesterase family member 5, ENPP5_HUMAN, NPP-5, UNQ550/PRO1107
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-ENPP5 Antibody [ARC2780] (A305501) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-ENPP5 Antibody [ARC2780] (A305501)
Western blot analysis of extracts of various cell lines, using Anti-ENPP5 Antibody [ARC2780] (A305501) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-ENPP5 Antibody [ARC2780] (A305501) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-ENPP5 Antibody [ARC2780] (A305501)
Western blot analysis of extracts of various cell lines, using Anti-ENPP5 Antibody [ARC2780] (A305501) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunofluorescence - Anti-ENPP5 Antibody [ARC2780] (A305501) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-ENPP5 Antibody [ARC2780] (A305501)
Immunofluorescence analysis of Neuro-2a cells using Anti-ENPP5 Antibody [ARC2780] (A305501) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-ENPP5 Antibody [ARC2780] (A305501) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-ENPP5 Antibody [ARC2780] (A305501)
Immunofluorescence analysis of SH-SY5Y cells using Anti-ENPP5 Antibody [ARC2780] (A305501) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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