Anti-Ephrin B2 Antibody [ARC0576] (A80671)

Name: Anti-Ephrin B2 Antibody [ARC0576]
Brand: Antibodies.com
SKU: A80671
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0576] antibody to Ephrin B2 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Ephrin B2 Antibody [ARC0576]

Description

Rabbit monoclonal [ARC0576] antibody to Ephrin B2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Ephrin B2 (P52799).

Sequence

MAVRRDSVWKYCWGVLMVLCRTAISKSIVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKDQADRCTIKKENTPLLN

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0576

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EFN B2, EFNB 2, Efnb2, EFNB2_HUMAN, Eph related receptor tyrosine kinase ligand 5, EPH-related receptor tyrosine kinase ligand 5, Ephrin-B2, EphrinB2, EPLG 5, EPLG5, Htk L, HTK ligand, HTK-L, HTKL, LERK 5, LERK-5, LERK5, Ligand of eph related kinase 5, MGC126226, MGC126227, MGC126228, OTTMUSP00000024973

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Ephrin B2 Antibody [ARC0576] (A80671)

Western blot analysis of extracts of various cell lines, using Anti-Ephrin B2 Antibody [ARC0576] (A80671) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Western Blot - Anti-Ephrin B2 Antibody [ARC0576] (A80671)

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Figure 3: Immunohistochemistry - Anti-Ephrin B2 Antibody [ARC0576] (A80671)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-Ephrin B2 Antibody [ARC0576] (A80671) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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