Anti-epithelial Sodium Channel alpha Antibody (A11846)

Name: Anti-epithelial Sodium Channel alpha Antibody
Brand: Antibodies.com
SKU: A11846
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to epithelial Sodium Channel alpha for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-epithelial Sodium Channel alpha Antibody

Description

Rabbit polyclonal antibody to epithelial Sodium Channel alpha.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 105-225 of human SCNN1A (NP_001029.1).

Sequence

LFGEYFSYPVSLNINLNSDKLVFPAVTICTLNPYRYPEIKEELEELDRITEQTLFDLYKYSSFTTLVAGSRSRRDLRGTLPHPLQRLRVPPPPHGARRARSVASSLRDNNPQVDWKDWKIG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

75 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Alpha ENaC, Alpha ENaC 2, Alpha NaCH, Alpha-ENaC, Alpha-NaCH, Amiloride sensitive epithelial sodium channel alpha subunit, Amiloride sensitive sodium channel subunit alpha, Amiloride-sensitive sodium channel subunit alpha, ENaCA, ENaCalpha, Epithelial Na(+) channel subunit alpha, Epithelial Na+ channel subunit alpha, FLJ21883, Nonvoltage gated sodium channel 1 subunit alpha, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, SCNN 1, SCNN1, Scnn1a, SCNNA_HUMAN, Sodium channel nonvoltage gated 1 alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-epithelial Sodium Channel alpha Antibody (A11846)

Western blot analysis of extracts of various cell lines, using Anti-epithelial Sodium Channel alpha Antibody (A11846) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunofluorescence - Anti-epithelial Sodium Channel alpha Antibody (A11846)

Immunofluorescence analysis of PC-12 cells using Anti-epithelial Sodium Channel alpha Antibody (A11846) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-epithelial Sodium Channel alpha Antibody (A11846)

Immunofluorescence analysis of NIH/3T3 cells using Anti-epithelial Sodium Channel alpha Antibody (A11846) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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