Anti-ERK1 + ERK2 Antibody [ARC51163] (A305894)

Name: Anti-ERK1 + ERK2 Antibody [ARC51163]
Brand: Antibodies.com
SKU: A305894

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This product is discontinued

Anti-ERK1 + ERK2 Antibody [ARC51163] (A305894) has been discontinued and is no longer available.

View all Anti-ERK1 + ERK2 Antibodies.

Specifications

Name

Anti-ERK1 + ERK2 Antibody [ARC51163]

Description

Rabbit monoclonal [ARC51163] antibody to ERK1 + ERK2.

Applications

WB, IHC

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK1/2ERK1/2 (P27361/P28482).

Sequence

FLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSD/LNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51163

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ERK 1, ERK 2, ERK-2, ERK1, erk1/2, ERK2, ERT1, ERT2, Extracellular signal regulated kinase 1, Extracellular signal-regulated kinase 2, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAP kinase isoform p44, MAPK 1, MAPK 2, MAPK 3, Mapk1, MAPK2, MAPK3, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, MK01_HUMAN, p38, p40, p41, p42-MAPK, PRKM 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-ERK1 + ERK2 Antibody [ARC51163] (A305894)

Western blot analysis of extracts of various cell lines, using Anti-ERK1 + ERK2 Antibody [ARC51163] (A305894) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-ERK1 + ERK2 Antibody [ARC51163] (A305894)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-ERK1 + ERK2 Antibody [ARC51163] (A305894) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-ERK1 + ERK2 Antibody [ARC51163] (A305894)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-ERK1 + ERK2 Antibody [ARC51163] (A305894) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-ERK1 + ERK2 Antibody [ARC51163] (A305894)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-ERK1 + ERK2 Antibody [ARC51163] (A305894) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.