Anti-ERK5 Antibody (A13879)

Name: Anti-ERK5 Antibody
Brand: Antibodies.com
SKU: A13879
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to ERK5 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-ERK5 Antibody

Description

Rabbit polyclonal antibody to ERK5.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 706-816 of human ERK5 (NP_002740.2).

Sequence

ESPDVNLVTQQLSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDGQADSASLSASLLADWLEGHGMNPADIESLQREIQMDSPMLLADLPDLQDP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

115 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Big MAP kinase 1, BMK 1, BMK 1 kinase, BMK-1, BMK1, BMK1 Kinase, EC 2.7.11.24, ERK 4, ERK 5, ERK-5, ERK4, Extracellular signal regulated kinase 5, Extracellular signal-regulated kinase 5, MAP kinase 7, MAPK 7, MAPK7, Mitogen activated protein kinase 7, Mitogen-activated protein kinase 7, MK07_HUMAN, OTTHUMP00000065906, OTTHUMP00000065907, PRKM 7, PRKM7, PROTEIN KINASE, MITOGEN-ACTIVATED, 7

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-ERK5 Antibody (A13879)

Western blot analysis of extracts of various cell lines, using Anti-ERK5 Antibody (A13879) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-ERK5 Antibody (A13879)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-ERK5 Antibody (A13879) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-ERK5 Antibody (A13879)

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-ERK5 Antibody (A13879) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.