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Anti-ERLIN1 Antibody (A89706)

Western Blot - Anti-ERLIN1 Antibody (A89706) - Antibodies.com Immunohistochemistry - Anti-ERLIN1 Antibody (A89706) - Antibodies.com Western Blot - Anti-ERLIN1 Antibody (A89706) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to ERLIN1 for WB, IHC and IP.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-ERLIN1 Antibody
Description
Rabbit polyclonal antibody to ERLIN1.
Applications
WB, IHC, IP
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 259-348 of human ERLIN1 (NP_006450.2).
Sequence
KADAEYYAAHKYATSNKHKLTPEYLELKKYQAIASNSKIYFGSNIPNMFVDSSCALKYSDIRTGRESSLPSKEALEPSGENVIQNKESTG
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
39 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Band 7 23-211 Keo4 (Interim) similar to C.elegans protein, C10orf69, chromosome 10 open reading frame 69, Endoplasmic reticulum lipid raft-associated protein 1, ER lipid raft associated 1, Erlin 1, Erlin-1, ERLN1_HUMAN, KE04, KEO4, OTTHUMP00000059425, Protein KE04, SPFH domain containing protein 1, SPFH domain family, member 1, SPFH domain-containing protein 1, SPFH1, SPG62, Stomatin-prohibitin-flotillin-HflC/K domain containing protein 1, Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-ERLIN1 Antibody (A89706) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-ERLIN1 Antibody (A89706)
Western blot analysis of extracts of various cell lines, using Anti-ERLIN1 Antibody (A89706) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-ERLIN1 Antibody (A89706) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-ERLIN1 Antibody (A89706)
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-ERLIN1 Antibody (A89706) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot - Anti-ERLIN1 Antibody (A89706) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-ERLIN1 Antibody (A89706)
Immunoprecipitation analysis of 300µg extracts of DU145 cells using 3µg of Anti-ERLIN1 Antibody (A89706). This Western blot was performed on the immunoprecipitate using Anti-ERLIN1 Antibody (A89706) at a dilution of 1:1000.
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