Anti-Estrogen Inducible Protein pS2 Antibody (A13656)

Western blot analysis of various lysates, using Anti-Estrogen Inducible Protein pS2 Antibody (A13656) at 1:700 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

Western blot analysis of Mouse stomach, using Anti-Estrogen Inducible Protein pS2 Antibody (A13656) at 1:700 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Estrogen Inducible Protein pS2 Antibody (A13656) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Estrogen Inducible Protein pS2 Antibody (A13656) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.