Anti-FACL4 Antibody [ARC53209] (A306923)

Name: Anti-FACL4 Antibody [ARC53209]
Brand: Antibodies.com
SKU: A306923
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC53209] antibody to FACL4 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-FACL4 Antibody [ARC53209]

Description

Rabbit monoclonal [ARC53209] antibody to FACL4.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:2,000-1:4,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 42-321 of human ACSL4 (NP_075266.1).

Sequence

MAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53209

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

79 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ACS 4, ACS4, ACSL 4, Acsl4, ACSL4_HUMAN, acyl CoA synthetase 4, Acyl CoA synthetase long chain family member 4, FACL 4, Fatty acid Coenzyme A ligase, fatty acid Coenzyme A ligase long-chain 4, LACS 4, LACS4, Lignoceroyl CoA synthase, Long chain 4, long chain acyl CoA synthetase 4, long chain fatty acid CoA ligase 4, long chain fatty acid Coenzyme A ligase 4, Long-chain acyl-CoA synthetase 4, Long-chain-fatty-acid--CoA ligase 4, MRX63, MRX68

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (10)

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Figure 1: Western Blot - Anti-FACL4 Antibody [ARC53209] (A306923)

Western blot analysis of extracts of various lysates, using Anti-FACL4 Antibody [ARC53209] (A306923) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 0. 3s.

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Figure 2: Western Blot - Anti-FACL4 Antibody [ARC53209] (A306923)

Western blot analysis of extracts of Rat liver, using Anti-FACL4 Antibody [ARC53209] (A306923) at 1:24000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-FACL4 Antibody [ARC53209] (A306923)

Western blot analysis of extracts of Mouse liver, using Anti-FACL4 Antibody [ARC53209] (A306923) at 1:24000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 4: Immunohistochemistry - Anti-FACL4 Antibody [ARC53209] (A306923)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-FACL4 Antibody [ARC53209] (A306923) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 5: Immunohistochemistry - Anti-FACL4 Antibody [ARC53209] (A306923)

Immunohistochemistry analysis of paraffin-embedded human lung using Anti-FACL4 Antibody [ARC53209] (A306923) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 6: Immunohistochemistry - Anti-FACL4 Antibody [ARC53209] (A306923)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-FACL4 Antibody [ARC53209] (A306923) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 7: Immunohistochemistry - Anti-FACL4 Antibody [ARC53209] (A306923)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-FACL4 Antibody [ARC53209] (A306923) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 8: Immunohistochemistry - Anti-FACL4 Antibody [ARC53209] (A306923)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-FACL4 Antibody [ARC53209] (A306923) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 9: Immunofluorescence - Anti-FACL4 Antibody [ARC53209] (A306923)

Confocal imaging of HepG2 cells using Anti-FACL4 Antibody [ARC53209] (A306923), at a dilution of 1:1000, (red). DAPI was used for nuclear staining (Blue). Objective: 60x.

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Figure 10: Western Blot - Anti-FACL4 Antibody [ARC53209] (A306923)

Immunoprecipitation analysis of 300µg extracts of HepG2 cells using 3µg of Anti-FACL4 Antibody [ARC53209] (A306923). This Western blot was performed on the immunoprecipitate using Anti-FACL4 Antibody [ARC53209] (A306923) at a dilution of 1:1000.

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