Fluorescein (FAM) is a popular fluorophore that has bright emission in the green area of visible spectrum. This derivative of fluorescein contains tetrazine moiety. Tetrazines react with trans-cycloalkenes and other strained olefins in a reaction called inverse electron demand Diels-Alder reaction (ied-DA). The reaction is very quick and specific. The reagent contains pure 6-isomer of FAM.
Absorption Maxima
490 nm
Extinction Coefficient
80000 M-1cm-1
Emission Maxima
513 nm
Fluorescence Quantum Yield
0.93
CF260
0.20
CF280
0.17
Mass Spec M+ Shift after Conjugation
531.1
Purity
95% (by 1H NMR and HPLC-MS).
Molecular Formula
C31H21N5O6
Molecular Weight
559.53 kDa
Product Form
Orange solid.
Solubility
Good in DMF and DMSO. Poor in water.
Storage
Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
