Rabbit polyclonal antibody to FANCA (phospho Ser1149).
Specificity
This antibody detects endogenous levels of FANCA only when phosphorylated at Ser1149.
Applications
WB, IHC, IF, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:1000
Reactivity
Human,
Immunogen
Synthetic peptide derived from human FANCA around the phosphorylation site of Ser1149 (amino acids 1121-1170).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
162kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
FA, FA 1, FA H, FA1, FAA, FACA, FAH, FANCA_HUMAN, FANCH, Fanconi anemia complementation group A, Fanconi anemia complementation group H, Fanconi anemia group A protein, Fanconi anemia type 1, MGC75158, Protein FACA
Figure 1: Western Blot - Anti-FANCA (phospho Ser1149) Antibody (A93805)
Western blot analysis of lysates from HeLa cells treated with IGF 100ng/ml 10' and HUVEC cells treated with EGF 200ng/ml 30' using Anti-FANCA (phospho Ser1149) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Anti-FANCA (phospho Ser1149) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunofluorescence analysis of HeLa cells using Anti-FANCA (phospho Ser1149) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
