Western blot analysis of extracts from normal (control) and FASN knockout (KO) 293T cells, using Anti-FASN Antibody (A0462) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Immunoprecipitation analysis of 200µg extracts of H460 cells, using 3µg Anti-FASN Antibody (A0462). Western blot was performed from the immunoprecipitate using Anti-FASN Antibody (A0462) at a dilition of 1:1000.