Anti-FAT1 Antibody [FAT1-3D7/1] (A248527)

Anti-FAT1 Antibody [FAT1-3D7/1]

Mouse monoclonal [FAT1-3D7/1] antibody to FAT1.

The FAT proteins are members of the Cadherin superfamily homologous to the Drosophila Fat protein that functions as a positive regulator of planar cell polarity in the Drosophila wing. FAT1 is an unusual cadherin that controls cell growth and planar polarity while acting as a tumor suppressor. FAT1 is a proximal element of a signaling pathway that determines both cellular polarity in the plane of the monolayer and directed actin-dependent cell motility. FAT1 is localized at the leading edge of lamellipodia, filopodia and microspike tips where it directly interacts with Ena/VASP proteins to regulate the actin polymerization complex. When targeted to mitochondrial outer leaflets, the cytoplasmic domain of FAT1 recruits components of the actin polymerization machinery sufficient to induce ectopic actin polymerization. FAT1 expression in vascular smooth muscle cells (VSMCs) increases significantly after arterial injury or growth factor stimulation, implicating FAT1 in the control of VSMC functions central to vascular remodeling by facilitating migration and limiting proliferation. FAT1 is also involved in psychic disorders, and its action may be of patho-physiological importance.

ELISA, WB, IF

WB: 1-2 µg/ml, IF: 1-2 µg/ml

Drosophila melanogaster

This antibody is predicted to cross-react with Chimpanzee and Rat due to sequence homology; 92% and 85% identity, respectively.

Cytoplasmic domain of drosophila FAT protein.

Mouse

Monoclonal

FAT1-3D7/1

IgM

Unconjugated

Protein A/G chromatography.

200 µg/ml

500 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-FAT1 Antibody [FAT1-3D7/1] - BSA and Azide free (A254301).

Cadherin family member 7, Cadherin family member 7 precursor, Cadherin ME5, Cadherin related tumor suppressor, Cadherin related tumor suppressor homolog, Cadherin related tumor suppressor homolog precursor, Cadherin related tumor suppressor homolog precursor (FAT protein homolog), Cadherin-related tumor suppressor homolog, CDHF 7, CDHF7, FAT, FAT 1, FAT tumor suppressor 1, FAT tumor suppressor homolog 1, FAT1_HUMAN, hFAT, hFat 1, hFat1, Homolog of Drosophila FAT protein, Homolog of Drosophila FAT protein precursor, Homolog of Drosophila tumor suppressor FAT precursor, ME 5, ME5, nuclear form, Protein fat homolog, Protocadherin Fat 1