Anti-FAT10 Antibody [ARC1379] (A306378)

Name: Anti-FAT10 Antibody [ARC1379]
Brand: Antibodies.com
SKU: A306378
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1379] antibody to FAT10 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-FAT10 Antibody [ARC1379]

Description

Rabbit monoclonal [ARC1379] antibody to FAT10.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 70-150 of human FAT10/UBD (O15205).

Sequence

KEKTIHLTLKVVKPSDEELPLFLVESGDEAKRHLLQVRRSSSVAQVKAMIETKTGIIPETQIVTCNGKRLEDGKMMADYGI

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1379

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

22 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Diubiquitin, GABBR1, Ubd, UBD 3, UBD_HUMAN, Ubiquitin D, Ubiquitin like protein FAT10, Ubiquitin-like protein FAT10

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-FAT10 Antibody [ARC1379] (A306378)

Western blot analysis of extracts of various cell lines, using Anti-FAT10 Antibody [ARC1379] (A306378) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunofluorescence - Anti-FAT10 Antibody [ARC1379] (A306378)

Immunofluorescence analysis of C6 cells using Anti-FAT10 Antibody [ARC1379] (A306378) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-FAT10 Antibody [ARC1379] (A306378)

Immunofluorescence analysis of NIH-3T3 cells using Anti-FAT10 Antibody [ARC1379] (A306378) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-FAT10 Antibody [ARC1379] (A306378)

Immunofluorescence analysis of U-2 OS cells using Anti-FAT10 Antibody [ARC1379] (A306378) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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